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Integrated consulting across biology, data and AI

Specialized Bio-Consulting Services

Detailed scientific and computational support for plasmids, vectors, strains, mRNA constructs, sequence analysis, AI-bio validation, bioprocess development and technical due diligence.

Explore our core service areas

Each engagement is scoped around your scientific question, target system, data readiness, confidentiality requirements and desired deliverables.

Plasmids, Vectors & ConstructsStrain EngineeringmRNA & RNA EngineeringAI-Bio & BioinformaticsBioprocess & QCAdvisory & Due Diligence
01

Plasmid, Vector & Construct Design

Custom sequence architectures designed for expression, storage, transfer, genome editing and comparative variant evaluation.

Plasmid Design for Recombinant Expression

  • Promoter, operator, enhancer and terminator selection
  • Host-specific codon optimization and coding-sequence review
  • Ribosome-binding site or Kozak-sequence optimization
  • Signal peptide, secretion sequence and affinity-tag design
  • Protease-cleavage site placement
  • Origin-of-replication, copy-number and selection-marker strategy
  • Restriction-site engineering and modular cloning design
  • Repeat, secondary-structure and instability-risk screening

Typical deliverables: Annotated plasmid map, GenBank and FASTA files, design rationale, cloning strategy and construct-risk report.

Storage, Archival & Transfer Vector Design

  • Stable storage of synthetic or structurally complex DNA
  • Low-copy vectors for unstable or repetitive inserts
  • Shuttle vectors for use across multiple host systems
  • Golden Gate, Gibson, Gateway and modular transfer architectures
  • Intermediate vectors for multi-step assembly
  • Large-insert and bacterial artificial chromosome strategy review
  • Backbone simplification and propagation planning

Typical deliverables: Vector architecture, backbone recommendation, stability-risk analysis and annotated storage-vector map.

DNA & RNA Construct Variant Design

  • Promoter, UTR and coding-sequence variant generation
  • Domain deletion, substitution and fusion-protein architecture
  • Linker, tag and protease-cleavage configuration
  • Reporter-gene integration
  • Bicistronic, polycistronic, IRES and 2A peptide designs
  • Comparative construct scoring and ranked testing recommendations

Typical deliverables: Ranked construct variants, design matrix, annotated sequences and recommended test order.

CRISPR & Genome-Editing Construct Design

  • Guide RNA target selection and off-target assessment
  • Cas nuclease and editing-system selection considerations
  • Donor-template and homology-arm design
  • Knock-in, knockout and multiplex guide strategies
  • Base-editing and prime-editing design review
  • Editing-vector and validation strategy design

Typical deliverables: Guide shortlist, donor-template design, editing construct map and validation plan.

02

Strain Engineering & Expression Optimization

Design and advisory support for industrial hosts, recombinant production systems and stable expression workflows.

Bacterial Strain Modification & Optimization

  • Host-strain selection for the intended product
  • Knockout, knockdown and genomic-integration planning
  • Protease-deficient and toxic-protein expression strategies
  • Metabolic-burden reduction and pathway redirection
  • Plasmid stability and segregation assessment
  • Promoter-strength, induction-system and copy-number balancing
  • Selection-marker-free or auxotrophic selection concepts
  • Strain–vector compatibility and scale-up risk analysis

Typical deliverables: Strain-modification blueprint, pathway map, host–vector compatibility report and optimization roadmap.

Translation Efficiency & Protein-Expression Optimization

  • Codon adaptation and codon-pair review
  • Ribosome-binding site and Kozak optimization
  • mRNA secondary-structure and initiation-site analysis
  • Signal peptide and transmembrane-domain review
  • Fusion-partner, linker and cleavage-site design
  • Rare-codon and translation-stall identification
  • Host comparison and induction-strategy recommendations

Typical deliverables: Optimized coding sequence, expression-risk report and host-specific design recommendations.

03

mRNA & RNA Engineering

Computational design and review of mRNA templates for research, therapeutic development, vaccines, protein expression and cell engineering.

mRNA Construct Design & Optimization

  • Coding-sequence optimization
  • 5′ and 3′ UTR design and comparative evaluation
  • Kozak-sequence optimization
  • Cap-strategy and poly(A)-tail design considerations
  • GC-content and secondary-structure balancing
  • Cryptic splice-site and premature polyadenylation screening
  • Innate-immune activation and instability-risk review
  • Template architecture for in vitro transcription
  • Plasmid-template design for mRNA production

Typical deliverables: Optimized mRNA sequence, alternative UTR variants, structural-risk report and annotated template map.

Primer, Probe & Molecular Assay Design

  • PCR, qPCR and RT-qPCR primer design
  • Sequencing, genotyping, cloning and mutagenesis primers
  • Hydrolysis probes, molecular beacons and hybridization probes
  • Melting-temperature and multiplex compatibility review
  • Specificity, dimer and off-target screening
  • Amplicon selection and assay-control recommendations

Typical deliverables: Primer and probe sequences, assay layout, specificity report and validation recommendations.

04

AI-Bio Validation & Bioinformatics

Independent biological oversight for AI-generated outputs, datasets, models and sequence-analysis workflows.

AI-Bio Model Validation & Domain Oversight

  • Biological plausibility and constraint-based assessment
  • Sequence, protein, RNA, plasmid and pathway output review
  • Hallucination and contradiction detection
  • Dataset-label and training-data quality review
  • Benchmark and validation-set design
  • Domain-expert annotation and reinforcement-learning feedback
  • Failure-mode and experimental-feasibility analysis
  • Human-in-the-loop validation framework design

Typical deliverables: Validation scorecard, annotated outputs, error taxonomy, benchmark recommendations and expert-review report.

Bioinformatics & Sequence Analysis

  • DNA and RNA sequence-quality review
  • Multiple-sequence alignment and variant identification
  • Motif, ORF, GC-content and repeat analysis
  • Restriction-map and plasmid-backbone review
  • Secondary-structure and codon-usage analysis
  • Regulatory-element annotation
  • Comparative construct analysis
  • Sequence-file standardization and documentation

Typical deliverables: Annotated sequence report, alignment files, variant table and sequence-quality summary.

05

Bioprocess, Scale-Up & Quality Control

Technical advisory to connect construct and strain designs with practical production, automation and validation workflows.

Bioprocess & Scale-Up Advisory

  • Host and expression-system selection
  • Fermentation and induction-strategy review
  • Feed strategy and cell-density trade-off assessment
  • Plasmid-maintenance and process-bottleneck analysis
  • Cell-free DNA and RNA production workflow review
  • Upstream-process and scale-dependent stability assessment
  • Automation-script and laboratory-constraint auditing
  • Process-transfer documentation

Typical deliverables: Process blueprint, scale-up risk register, workflow diagram and optimization roadmap.

Quality Control & Validation Architecture

  • Construct identity and sequence-verification planning
  • Plasmid-integrity and expression-confirmation strategies
  • RNA cap, poly(A)-tail and integrity assessment planning
  • Probe-based assay and NGS validation design
  • Acceptance-criteria and control-material recommendations
  • Batch-comparison and root-cause analysis frameworks
  • Quality-risk assessment and reporting templates

Typical deliverables: QC workflow, validation matrix, acceptance criteria, assay plan and reporting template.

06

Scientific Advisory & Technical Due Diligence

Independent scientific review and flexible expert support for biotech, biopharma, AI-bio, academic and industrial biology teams.

Technical Due Diligence & Independent Review

  • Construct and platform feasibility assessment
  • Scientific-claim and technology-risk review
  • Data-quality and experimental-plan evaluation
  • Development-roadmap and vendor deliverable review
  • Technical red-flag identification
  • Pre-investment scientific assessment
  • Competitive technical landscape review

Typical deliverables: Due-diligence report, risk matrix, executive summary and recommendation memo.

Scientific Strategy & Project Advisory

  • Research-program and experimental-priority planning
  • Construct-development and platform-selection strategy
  • Technical troubleshooting and literature review
  • Vendor and CRO evaluation
  • Scientific presentation, grant and proposal review
  • Project milestone and technical roadmap development
  • Advisory meetings and team workshops

Typical deliverables: Advisory memo, project roadmap, decision matrix, workshop materials and prioritized action plan.

Need a tailored combination of services?
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